The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids.

Telomerase is an enzyme that adds repetitive DNA sequences to the ends of chromosomes and consists of two main subunits: the telomerase reverse transcriptase (TERT) protein and an associated telomerase RNA (TER). The telomerase essential N-terminal (TEN) domain is a conserved region of TERT proposed to mediate DNA substrate interactions. Here, we have employed single molecule telomerase binding assays to investigate the function of the TEN domain. Our results reveal telomeric DNA substrates bound to telomerase exhibit a dynamic equilibrium between two states: a docked conformation and an alternative conformation. The relative stabilities of the docked and alternative states correlate with the number of basepairs that can be formed between the DNA substrate and the RNA template, with more basepairing favoring the docked state. The docked state is further buttressed by the TEN domain and mutations within the TEN domain substantially alter the DNA substrate structural equilibrium. We propose a model in which the TEN domain stabilizes short RNA-DNA duplexes in the active site of the enzyme, promoting the docked state to augment telomerase processivity

Electrostatic interactions between discrete helices of charge

We analytically examine the pair interaction for parallel, discrete helices of charge. Symmetry arguments allow for the energy to be decomposed into a sum of terms, each of which has an intuitive geometric interpretation. Truncated Fourier expansions for these terms allow for accurate modeling of both the axial and azimuthal terms in the interaction energy and these expressions are shown to be insensitive to the form of the interaction. The energy is evaluated numerically through application of an Ewald-like summation technique for the particular case of unscreened Coulomb interactions between the charges of the two helices. The mode structures and electrostatic energies of flexible helices are also studied. Consequences of the resulting energy expressions are considered for both F-actin and A-DNA aggregates

In an expanding universe, what doesn't expand?

The expansion of the universe is often viewed as a uniform stretching of space that would affect compact objects, atoms and stars, as well as the separation of galaxies. One usually hears that bound systems do not take part in the general expansion, but a much more subtle question is whether bound systems expand partially. In this paper, a very definitive answer is given for a very simple system: a classical "atom" bound by electrical attraction. With a mathemical description appropriate for undergraduate physics majors, we show that this bound system either completely follows the cosmological expansion, or -- after initial transients -- completely ignores it. This "all or nothing" behavior can be understood with techniques of junior-level mechanics. Lastly, the simple description is shown to be a justifiable approximation of the relativistically correct formulation of the problem.Comment: 8 pages, 9 eps figure

New State Record and Notable Range Extension for \u3ci\u3eLibellula Semifasciata\u3c/i\u3e (Odonata: Libellulidae)

The painted skimmer, Libellula semifasciata Burmeister (Odonata: Libellulidae), is an eastern species of dragonfly that has never been documented in Iowa. In this note we report two observations and the collection of a voucher for this species in southeast Iowa in the last three years. Based on other records of this species, including those from neighboring states and more northerly latitudes, we propose that these observations are evidence of a range extension

Convex hull method for the determination of vapour-liquid equilibria (VLE) phase diagrams for binary and ternary systems

Amieibibama Joseph wishes to thank Petroleum Technology Development Fund (PTDF) for their financial support which has made this research possible.Peer reviewedPostprin

Sky survey at far infrared wavelengths using a balloon-borne telescope

Localized sources of far infrared radiation (approximately 50 microns) have been detected during a high altitude balloon flight with a 40 cm telescope and silicon detectors. The flight system is described and preliminary results are presented. A large area of the sky has been scanned for localized sources of far infrared radiation, using a balloon-borne system that was sensitive to wavelengths beyond about 55 microns. Two Molectron silicon bolometers were used, with a Newtonian telescope having a 40 cm primary. The telescope was driven in azimuth at a fixed elevation; this mode of scanning was carried out for the duration of each of two balloon flights. The flight system is described

ESTIMATING RETURNS TO AGRICULTURAL RESEARCH, EXTENSION, AND TEACHING AT THE STATE LEVEL

The majority of decisions concerning investment and allocation of public funds for agricultural research, extension, and teaching (RET) are made at the state-level, while most of the quantitative RET evaluations are made on a national basis. This paper illustrates an approach for conducting a disaggregated state-level evaluation of agricultural research, extension, and teaching. Ridge regression is employed to handle multicollinearity problems.Teaching/Communication/Extension/Profession,

Driving forces in free visual search : An ethology

Peer reviewedPostprin